ntera2 d1 hnt nt2 cell line  (ATCC)

Journal: Biosensors

Article Title: Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time

doi: 10.3390/bios5020199

Figure Lengend Snippet: Optimisation of ideal culture conditions. Different Cell Index adhesion curves are obtained from different cells types. The adhesion of ( a ) HMEC-1 endothelial cells and ( b ) NTera2/D1 (NT2)-astrocytes is shown. Both cell types were seeded at a range of titrations to identify the ideal seeding density and reveal time windows for drug treatment. Curves represent the mean Cell Index value from >4 wells ± SD.

Article Snippet: The NTera2/D1 (NT2) cell line was purchased from ATCC (American Tissue Culture Collection).

Techniques:

Journal: Biosensors

Article Title: Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time

doi: 10.3390/bios5020199

Figure Lengend Snippet: Direct comparison of growth characteristics of NT2-astrocytes from different cultures. Here, xCELLigence was used to assess the consistency and growth characteristics of NT2-derived astrocytes produced from different differentiations (and by different students). This example shows that the astrocytes in Differentiation C had a very low Cell Index, which is inconsistent with the strong level of adhesion typical of these cells (Differentiations A and B).

Article Snippet: The NTera2/D1 (NT2) cell line was purchased from ATCC (American Tissue Culture Collection).

Techniques: Comparison, Derivative Assay, Produced

Journal: Biosensors

Article Title: Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time

doi: 10.3390/bios5020199

Figure Lengend Snippet: Use of xCELLigence for measuring drug effects on cell viability, compromise and death. Here, NT2 astrocytes were treated with the pro-inflammatory cytokine IL1β at a range of concentration to assess the acute and long-term effects on the cells. During the initial 24-h period following cytokine treatment, there is a pronounced increase in the Cell Index, which is consistent with inflammatory activation of the cell and increased astrocytic size as a consequence. This is followed by a progressive and continual loss of adhesion. IL1β was added 24 h after seeding, and each curve represents the mean ± SD of four wells. The images in ( b ) show the viable astrocytes remaining on the E-plate at the end of the experiment. The white dotted circles reveal the position of the non-transparent electrode array. These images were acquired using an inverted microscope. The array can be seen more easily in the bright-field insert. Note that ACEA have developed plates with view strips for imaging of cells during xCELLigence experiments.

Article Snippet: The NTera2/D1 (NT2) cell line was purchased from ATCC (American Tissue Culture Collection).

Techniques: Concentration Assay, Activation Assay, Inverted Microscopy, Imaging